产品说明
一般描述
This Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) is validated for use in Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of IGF-IR (Ab-1).
Purified mouse monoclonal antibody generated by immunizing SJL mice with the specified immunogen and fusing splenocytes with FO mouse myeloma cells (see application references). Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
免疫原
partially purified, human placenta IGF-I receptor
Human
包装
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
Immunoblotting (not recommended)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Neutralization Studies (1 µg/ml; use Cat. No. GR11L)
Paraffin Sections (not recommended)
警告
Toxicity: Standard Handling (A)
外形
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
分析说明
Positive Control
HepG2 cells
Negative Control
HS27 cells
其他说明
Roth, R. 1988. Science239, 1269.
Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm.149, 276.
Rosen, O.M., 1987. Science257, 1452.
Rechler, M.M. and Nissley, S.P., 1986. Hormone Res.24, 152.
Ullrich, A., et al. 1986. EMBO J.5, 2503.
Zapf, S. and Froesch, E.R., 1986. Hormone Res.24, 121.
Humbel, R.E., 1984. I. Chemistry; in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York).
Kull, F.C., et al. 1983. J. Biol. Chem.258, 6561.
Anti-IGF-I Receptor (Ab-1) Mouse mAb (αIR3) immunoprecipitates the α and β subunits of the IGF-I receptor. Blocks IGF-I binding to its receptor and may bind weakly to the insulin receptor. It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in indvidual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | αIR3, monoclonal |
形式 | liquid |
包含 | ≤0.1% sodium azide as preservative |
species reactivity | human |
manufacturer/tradename | Calbiochem® |
储存条件 | do not freeze |
同位素/亚型 | IgG1 |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich