Anti-Homer1 Antibody

产品说明

一般描述

Homer protein homolog 1 (UniProt Q86YM7; also known as Homer-1, Homer1) is encoded by the HOMER1 (also known as SYN47) gene (Gene ID 9456) in human. Homer-1, -2, and -3 constitute a family of postsynaptic scaffold proteins that play a central role in calcium signaling. Homer members share a conserved N-terminal Ena/VASP homology 1 (EVH1) domain (a.a. 1-110 of Homer-1) that mediate signaling complexes assembly by interacting with PPXXFR proline-rich consensus sequences found in various other scaffolding and signal transduction molecules, including metabotropic glutamate receptors (mGluRs) and 1,4,5-trisphosphate receptors (IP3Rs). In addition to mediate group I mGluRs signaling via their EVH1 domain, Homer-1 spliced variants Vesl-1L, 1b, and 1d, but not Vesl-1M or Vesl-1S, also contain a C-terminal coiled-coil (a.a. 181-352) region that mediate self-multimerization. Homer-1 variants are associated with the etiology of many neurological diseases, such as chronic pain, mental retardation syndromes, Alzheimer′s disease (AD), Parkinson′s disease (PD), schizophrenia, drug-induced addiction, and traumatic brain injury (TBI). Homer1b/c-knockdown is reported to protect against chemically induced seizure in mice as a result of preventing mGluR5-mediated mTOR pathway activation.

特异性

Broad species reactivity expected based on high sequence homology.
This rabbit polyclonal antibody targets an internal sequence within the N-terminal half of human Homer1 isoform 1b and mouse isoform Vesl-1L. Immunogen sequence appears near the C-terminus of human isoform 1h and mouse isoforms 1b, 1d, Vesl-1M, and Vesl-1S, but absent in human isoform 1e. Highly homologous sequence (~83%) is also found in the N-terminal half of rat isoform 1c (Vesl-1L) and near the C-terminus of rat isoforms 1a (Vesl) and 1b.

免疫原

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human Homer1 isoform 1b and near the C-terminus of isoform 1h.

应用

Immunohsitochemistry Analysis: A 1:500 dilution from a representative lot detected Homer1 in human and mouse cerebral cortex tissue sections.

Immunohsitochemistry Analysis: A representative lot detected significantly increased stratum radiatum and stratum oriens Homer1 immunoreactivity in floating brain sections from mice received AP-12 intraperitoneal injection (Jansone, B., et al. (2015). PLoS One. 10(6):e0127686).

Anti-Homer1, Cat. No. ABN37, is a highly specific rabbit polyclonal antibody that targets Homer protein homolog 1 and has been tested in Immunohistochemistry and Western Blotting.

质量

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Homer1 in 10 µg of mouse brain tissue lysate.

目标描述

~47 kDa observed. 40.28/19.86 kDa (human isoform 1b/1h), 40.22/42.08/41.41/22.78/20.91 kDa (mouse isoform 1b/1d/Vesl-1L/Vesl-1M/Vesl-1S) calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Affinity purified.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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