产品说明
应用
L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection .
包装
100 units in glass bottle
生化/生理作用
L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.
单位定义
One unit will convert 1.0 μmole of
外形
Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7
基本信息
| 酶学委员会(EC)编号 | 1.4.1.1 ( BRENDA | IUBMB ) |
| EC 号 | 232-847-9 |
| MDL编号 | MFCD00131441 |
| NACRES | NA.54 |
产品性质
| 形式 | buffered aqueous glycerol solution |
| 质量水平 | 200 |
| specific activity | ~30 units/mg protein (Lowry) |
| 异质活性 | LDH ~1% (using pyruvate as substrate) |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H334 |
| 预防措施声明 | P261 - P284 - P501 |
| 危险分类 | Resp. Sens. 1 |
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
