产品说明
一般描述
This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells. Available as a 500mM stock solution (1mL). A complete protocol is included with shipment.
应用
Sodium butyrate can act to increase the efficiency of transfection and expression for both transient and stable transfections. It increases enhancer-dependant transcription of inserts driven by the SV40 early promoter. Vectors of this type include any of the pSV and pSV2 plasmids. Treatment of transfected cells with sodium butyrate can result in: a 2-3 fold increase in the number of cells able to express the insert; a 10-30 fold increase in the number of stable transfected colonies; and cells treated with sodium butyrate at the time of transfection can be re-induced by a second treatment weeks after the initial treatment and yield a 4-7 fold increase in transcription of the SV40 early promoter driven insert.
This treatment can be used on cells transfected by the Calcium Phosphate, DEAE Dextran and electroportation method.
储存及稳定性
Store at 2-8°C for 2 years.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160801 |
| NACRES | NA.75 |
产品性质
| 质量水平 | 200 |
| 形式 | liquid |
| manufacturer/tradename | Specialty Media |
| input | sample type: mouse embryonic stem cell(s) |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H314 |
| 预防措施声明 | P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 |
| 危险分类 | Eye Dam. 1 - Skin Corr. 1B |
| 储存分类代码 | 8B - Non-combustible, corrosive hazardous materials |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
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