Anti-TGF-beta Receptor Antibody, type I

产品说明

一般描述

TGF-beta receptor type-1 (UniProt: P36897; also known as EC:2.7.11.30, TGFR-1, Activin A receptor type II-like protein kinase of 53kD, Activin receptor-like kinase 5, ALK-5, Serine/threonine-protein kinase receptor R4, SKR4, Transforming growth factor-beta receptor type I, TbetaR-I) is encoded by the TGFBR1 (also known as ALK5, SKR4) gene (Gene ID: 7046) in human. TGF-beta R1 is found in all tissues examined and is most abundant in placenta and least abundant in brain and heart. It is also expressed in a variety of cancer cell lines. TGF-beta signaling involves its binding to the TGF-beta receptor type II (TGF-beta RII), which allows it to recruit TGF-beta receptor type I (TGF-beta RI) and assemble it as a heterodimeric receptor complex. TGF-beta RII phosphorylates TGF-beta RI in the glycine-serine (GS) rich region and activates the serine/threonine kinase activity of TGF-beta RI, which in turn phosphorylates receptor-linked Smad (Small mothers against decapentaplegic) proteins. To prevent any spontaneous phosphorylation of Smads, the inhibitor molecule FKBP12 binds to the GS region of the TGF-beta RI and blocks the access of TGF-beta RII to this domain. This inhibitory effect is removed upon TGF-beta binding to the receptor.

特异性

This polyclonal antibody specifically detects TGF-beta receptor type 1 in multiple species. It targets an epitope of 15 amino acids in the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human TGF-beta receptor 1.

应用

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TGF-beta receptor type-1 in human spleen, human cerebellum, human kidney, human pancreas, human placenta (chorionic villi), and human placenta (decidual cells).

Flow Cytometry Analysis: 1 µg from a representative lot detected TGF-beta receptor type-1 in one million mouse splenocyte cells.

This rabbit polyclonal Anti-TGF-beta Receptor, type I, Cat. No. ABF17-I is tested in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of TGF-beta receptor type-1.

质量

Evaluated by Western Blotting in rat liver tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TGF-beta receptor type-1 in 10 µg of rat liver tissue lysate.

目标描述

~56 kDa observed, 55.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

联系

Replaces: ABF17

外形

Affinity Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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