Anti-Myeloperoxidase Antibody

产品说明

一般描述

Myeloperoxidase (EC 1.11.2.2; UniProt P05164) is encoded by the MPO gene (Gene ID 4353) in human. Myeloperoxidase/MPO is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a 745 a.a. single chain precursor with a signal peptide sequence (a.a. 1-48), myeloperoxidase is subsequently cleaved into a light chain (a.a.165-278) and a heavy chain (a.a. 279-745). The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. MPO catalyzes hydrogen peroxide-dependent oxidations and the formation of hypochlorous acid that is central to the microbicidal activity of netrophils.

特异性

The immunogen sequence is present in the pro-fom and the mature forms of myeloperoxidase (without a.a. 1-48, a.a. 1-154, or a.a. 1-164), as well as the cleaved heavy chain (a.a. 279-745), but not light chain (a.a. 165-278).

免疫原

KLH-conjugated linear peptide corresponding to a sequence within human myeloperoxidase heavy chain region.

应用

Flow Cytometry Analysis: 0.1 µg from a representative lot detected myeloperoxidase immunoreactivity in 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized HL-60 cells.

Anti-Myeloperoxidase Antibody, Cat. No. 07-496-I, is a highly specific rabbit polyclonal antibody that targets Myeloperoxidase and has been tested in Flow Cytometry and Western Blotting.

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected myeloperoxidase heavy chain in 10 µg of Jurkat cell lysate.

目标描述

~92/60 kDa (glycosylated pro-form/heavy chain) observed. 83.87/73.85/87.25 kDa (isoform H17/H14/H7 pro-form), 78.94/82.32 kDa (isoform H17/H7 with a.a. 1-48 propeptide removed), 66.45/66.45/69.83 kDa (isoform H17/H14/H7 with a.a. 1-154 removed), 65.48/65.48/68.86 kDa (isoform H17/H7 isoform H17/H14/H7 with a.a. 1-164 removed), 52.51 kDa (heavy chain; a.a. 279-745) calculated. Uncharacterized bands may be observed in some lysate(s).

联系

Replaces: 07-496

外形

Affinity purified.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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