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Anti-Importin β antibody,Mouse monoclonal

品牌
Sigma-Aldrich
货号
I2534
规格纯度
clone 31H4, purified from hybridoma cell culture
参考价格
5201.04 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Importin β antibody,Mouse monoclonal
Anti-p97
Anti-karyopherin β1
Anti-Imp β
产品介绍:

产品说明

一般描述

Importin β comprises 19 tandemly repeated Huntingtin, elongation factor 3 (EF3), protein phosphatase 2A (PP2A), and the yeast kinase serine/threonine-protein kinase-TOR1 (HEAT) motifs and wraps around the importin-β (IBB). It also consists of an N-terminal RAN guanosine triphosphate (GTP) binding domain, a middle zone interacting with the nuclear pore, and an importin α binding domain at the C-terminus. The importin β gene is mapped to human chromosome location 17q21.32.
Monoclonal Anti-Importin b (mouse IgG1 isotype) is derived from the 31H4 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with importin. Importin β (Imp β), also referred to as karyopherin β1, is an import receptor that belongs to the family of nuclear transport receptors.

特异性

Monoclonal Anti-Importin β recognizes human, bovine, rat, and mouse importin β.

免疫原

importin β

应用

Anti-Importin β antibody,Mouse monoclonal has been used in:

  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunofluorescent staining
  • coimmunoprecipitaion
  • western blotting
  • immunofluorescence
  • immunoprecipitation

生化/生理作用

Importin β (Impβ) forms a complex with importin α, which, in turn, binds the cargo protein via its NLS (nuclear localization signals). The cargo protein is transported by this ternary complex to the cytoplasmic face of the nuclear pore complex (NPC) via a direct interaction with specific nucleoporins. When the complex reaches the nuclear site of the nuclear pore complex (NPC), Ran-GTP binds to Impβ to form an Impβ/Ran-GTP complex and releases Impα and the cargo protein. The Impβ/Ran-GTP complex is then transported back to the cytoplasm, where the complex dissociates upon hydrolysis of GTP to GDP. Importin β is then ready to participate in new nuclear import cycle.
Importin β regulates cellular functions, such as mitotic spindle assembly and centrosome dynamics. It is implicated in the insertion of integral membrane proteins into the inner nuclear membrane. Importin β also functions as a chaperone for highly basic proteins in the cytoplasm.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD07785407
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆31H4, monoclonal
形式buffered aqueous solution
分子量antigen ~97 kDa
species reactivityrat, mouse, human, bovine
technique(s)immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa total cell extract
同位素/亚型IgG1
UniProt登记号Q14974
运输dry ice
储存温度−20℃
Gene Informationhuman ... KPNB1(3837)
mouse ... Kpnb1(16211)
rat ... Kpnb1(24917)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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