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位置:首页 > 品牌 > Sigma-Aldrich > Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312, Trial Size

Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312, Trial Size

品牌
Sigma-Aldrich
货号
05-1336-S
规格纯度
clone CMA312, Upstate®, from mouse
参考价格
1660.9 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312, Trial Size
H3S10P, Histone H3 (phospho Ser10), H3 Histone, family 3A
产品介绍:

产品说明

一般描述

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.

特异性

This antibody specifically recognizes Histone H3 phosphorylated at Ser10.
Broad species cross-reactivity is expected, based on sequence homology.

免疫原

Epitope: Phospho Ser10
Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, phosphorylated on Ser10, conjugated to KLH.

应用

Research Sub Category
Chromatin Biology

Histones
Research Category
Epigenetics & Nuclear Function
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312 is a mouse monoclonal antibody for detection of phospho-Histone H3 (Ser10) also known as H3S10P, Histone H3 (phospho S10) & has been validated in WB, ELISA, ICC, Multiplexing and ChIP.

质量

Western Blot Analysis: 1 μg/mL of this antibody detected phospho-Histone H3 (Ser10) on 1 μg of colcemid-treated HeLa acid extract.

目标描述

~17 kDa

外形

Protein G Chromatography
Format: Purified
Purified mouse monoclonal IgG in PBS with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
HeLa Acid extract lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆CMA312, monoclonal
species reactivityvertebrates, human
manufacturer/tradenameUpstate®
technique(s)ChIP: suitable
ELISA: suitable
immunocytochemistry: suitable
multiplexing: suitable
western blot: suitable
同位素/亚型IgG1
NCBI登记号NP_003484.1
UniProt登记号P84243
运输wet ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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