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位置:首页 > 品牌 > Sigma-Aldrich > Anti-PARP (214/215) cleavage site Antibody

Anti-PARP (214/215) cleavage site Antibody

品牌
Sigma-Aldrich
货号
AB3565
规格纯度
Chemicon®, from rabbit
参考价格
7304.09 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
Anti-PARP (214/215) cleavage site Antibody
Poly [ADP-ribose] polymerase 1, PARP-1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, ARTD1
产品介绍:

产品说明

一般描述

Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

特异性

Reactivity with other species has not been confirmed. This sequence is 86% homologous in mouse, rat, and hamster.
The antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

免疫原

Synthetic peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.

应用

Research Sub Category
Apoptosis - Additional

Enzymes & Biochemistry
Anti-PARP (214/215) cleavage site Antibody detects level of PARP (214/215) cleavage site & has been published & validated for use in WB & IC.
For Western blot applications, we recommend using the antibody at a 1:1,000 starting dilution.
Research Category
Apoptosis & Cancer

Metabolism

质量

Purified from rabbit serum by affinity chromatography using a peptide corresponding to the PARP cleavage site.

联系

Replaces: 04-575; 04-576

外形

Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide
ImmunoAffinity Purified

储存及稳定性

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

分析说明

Control
Jurkat and HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman, bovine, rat, mouse
manufacturer/tradenameChemicon®
technique(s)immunocytochemistry: suitable
western blot: suitable
NCBI登记号NM_001618.3
UniProt登记号P09874
运输dry ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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