产品说明
应用
Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 ℃. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 ℃ to 25 ℃, the pH decreases an average of 0.03 pH units per ℃.
From 25 ℃ to 37 ℃, the pH decreases an average of 0.025 pH units per ℃.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
该产品经细胞培养测试。它还经过了低内毒素水平检测并分析了是否已去除核酸酶和蛋白酶。
包装
简易锡箔袋包装
其他说明
This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.
某些pH电极不能使用Tris缓冲液提供准确的结果。
制备说明
Trizma溶液可高压灭菌。
法律信息
Trizma is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.25 |
产品性质
| 质量水平 | 200 |
| 等级 | BioPerformance Certified |
| 形式 | crystalline powder |
| 分子量 | average Mw 141.8 |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | DNase, RNase, Protease, NICKase, none detected endotoxin, tested total aerobic microbial count, tested ≤0.05 A290 ≤0.5% water (Karl Fischer) ≤5 ppm Heavy metals (as Pb) |
| pH值(酸碱度) | 7.71 (37 ℃, 0.1 M)(lit.) 8.00 (25 ℃, 0.1 M)(lit.) 8.0 8.58 (5 ℃, 0.1 M)(lit.) |
| 有效pH范围 | 7.0 - 9.0 |
| application(s) | diagnostic assay manufacturing |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | dust mask type N95 (US), Eyeshields, Gloves |
Sigma-Aldrich
m.cnreagent.com
