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Caspase 8 Colorimetric Activity Assay Kit 25, IETD

品牌
Sigma-Aldrich
货号
APT129
规格纯度
Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.
参考价格
4962.96 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Caspase 8 Colorimetric Activity Assay Kit 25, IETD
FLICE
产品介绍:

产品说明

一般描述

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.

应用

Research Category
Apoptosis & Cancer

组分

5X Cell Lysis Buffer (Part No. 90065): 5 mL

5X Assay Buffer (Part No. 90066): 10 mL

Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90081): 250 μL of 2.5 mg/mL solution

Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 mM (0.05 mg/mL) in DMSO

pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO

储存及稳定性

Store kit materials at -20°C up to their expiration date.

Special Precautions:

· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.

· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32161000
NACRESNA.84

产品性质

质量水平100
species reactivity (predicted by homology)mammals
manufacturer/tradenameChemicon®
technique(s)activity assay: suitable
NCBI登记号NM_001080124.1
NM_001080125.1
NM_001228.4
NM_033355.3
NM_033356.3
NM_033358.3
UniProt登记号Q14790
detection methodcolorimetric
运输dry ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

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