Kinase Assay Kit

产品说明

一般描述

Kinases, also known as phosphotransferases, constitute a large family of enzymes that transfer phosphate groups from the high-energy donor molecule ATP, to their specific substrates. Kinases are known to regulate the majority of cellular processes. The largest group of this family is the protein kinases. So far, 518 different kinases have been identified in humans and up to 30% of human proteins are modified by these kinases. The enormous diversity and their key role in cellular signaling make them ideal targets for drug developments.

特点和优势

• Sensitive and accurate. As low as 0.01 U/L kinase can be quantified.
• Convenient and fast. The procedure is performed at room temperature and involves adding a single working reagent followed by a 10 minute incubation.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for the determination of protein kinase activity and for high-throughput screening of kinase inhibitors.

原理

The Kinase Assay Kit provides a simple and rapid method for assaying kinase activity and high-throughput screening for kinase inhibitors. This homogeneous microplate-based assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted to ATP and pyruvate, which is quantified using a fluorometric (Ex = λ530 nm/λEm = 590 nm) assay method.

产品性质

安全信息