产品说明
一般描述
Human PARP1 (GenBank Accession No. NM_001618), full length with N-terminal GST tag, MW = 140 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in baculovirus infected insect cells |
| 测定 | ≥80% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 140 kDa |
| 包装 | pkg of 10 and 20 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | NM_001618 |
| UniProt登记号 | P09874 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... PARP1(142) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
