Anti-APC (Ab-1) Mouse mAb (FE9)

产品说明

一般描述

Anti-APC (Ab-1), mouse monoclonal, clone FE9, recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells. It is validated for Western botting.
Protein G purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP40 cells. Recognizes the ~300 kDa APC protein as well as a variety of truncated forms.
Recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells.

免疫原

a synthetic peptide corresponding to the N-terminal 35 amino acids of APC

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (1 µg/ml, see comments)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, pH 7.5, 0.2% gelatin.

分析说明

Positive Control
HCT116 cells for p300, SW480 cells for truncated APC (p147)

其他说明

Koetsier, P. A., et al. 1993. BioTechniques15, 258.
Smith, K. J., et al. 1993. Proc. Natl. Acad. Sci., USA90, 2846.
Su, L.-K., et al. 1993. Can. Res.53, 2728.
Boynton, R. F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 3385.
D′Amico, D., et al. 1992. Cancer Res.52, 1996.
Fearon, E. R., and Jones, P. A., 1992. FASEB J.6, 2783.
Miyoshi, Y., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4452.
Powell, S. M., et al. 1992. Nature359, 235.
Groden, J., et al. 1991. Cell66, 589.
Kinzler, K. W., et al. 1991. Science253, 661.
Nishisho, I., et al. 1991. Science253, 665.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息