Anti-N-Myc Mouse mAb (NCM II 100)

产品说明

一般描述

This Anti-N-Myc Mouse mAb (NCM II 100) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of N-Myc.
Recognizes the N-myc protein and its cleavage product in IMR5 cells.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc and its cleavage products.

免疫原

Human
recombinant, human, N-myc fusion protein, expressed in E. coli

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (1-5 µg/ml)

Immunoblotting (1-5 µg/ml, see application references)

Immunofluorescence (1-5 µg/ml, see application references)

Immunoprecipitation (1-2 µg/sample, see application references)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 50% glycerol.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
IMR5 cells

其他说明

It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
Brondyk, W.H. 1991. Oncogene6, 1269.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research.2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen.20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA83, 5929.
Slamon, D.J., et al. 1986. Science232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med.313, 1111.
Brodeur, G.M., et al. 1984. Science224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol.11, 2370.
Kohl, N.E., et al. 1983. Cell35, 603.
Schwab, M., et al. 1983. Nature305, 245.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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