产品说明
特异性
Recognizes AANAT (Arylalkylamine N-acetyltransferase). The antibody specifically recognizes the phosphorylated N-terminal PKA site of AANAT (pT31) (Ganguly et al. 2005). The antibody reacts with natural and recombinant protein.
免疫原
Synthetic peptide (phosphorylated) corresponding to amino acids near the N-terminous of rat AANAT conjugated to BSA. {Ganguly, S et al, 2005}.
应用
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Anti-AANAT Antibody is an antibody against AANAT for use in WB.
Western Blot: 1:10,000-1:20,000 using ECL. Reacts with the ~23 kDa AANAT protein. Some reactivity may also be seen at ~97 kDa. Suggested blocking buffer is PBS with 10% non fat dry milk and 0.2% Tween-20. Suggested dilution buffer is PBS with 1 mg/mL BSA fraction V. Suggested incubation time is overnight at room temperature (~23°C).
Optimal working dilutions must be determined by the end user.
外形
Liquid rabbit serum containing no preservative.
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
分析说明
Control
POSITIVE CONTROL:
Night adapted rat pineal gland. Expression is light sensitive; highest levels are obtained from animals at night.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human, fish, bovine, sheep, rat |
manufacturer/tradename | Chemicon® |
technique(s) | western blot: suitable |
NCBI登记号 | NM_001088.1 |
UniProt登记号 | Q16613 |
运输 | dry ice |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich