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Anti-Cathepsin G Antibody, clone AHN-11, Ascites Free

品牌
Sigma-Aldrich
货号
MABT1240
规格纯度
clone AHN-11, from mouse
参考价格
5426.21 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Cathepsin G Antibody, clone AHN-11, Ascites Free
Cathepsin G, CatG, CG
产品介绍:

产品说明

一般描述

Cathepsin G (EC 3.4.21.20; UniProt P08311; also known as CatG, CG) is encoded by the CTSG gene (Gene ID 1511) in human. Cathepsin G is a peptidase S1 protein family protease found in azurophil granules of neutrophilic polymorphonuclear leukocytes. Cathepsin G displays a substrate specificity similar to that of chymotrypsin C, but it is most closely related to other immune serine proteases, such as neutrophil elastase and the granzymes. Cathepsin G is involved in connective tissue remodeling at sites of inflammation. Cathepsin G is also a component of neutrophil extracellular traps (NETs) composed of ejected lattice of chromatin enmeshed with granular and nuclear proteins, including histones, calprotectin and cathepsin G, that are capable of capturing and killing microbial invaders. Antibodies raised against cathepsin G or histones have been shown to limit the bactericidal capacity of NETs towards pathogens. Cathepsin G is prouduced with a signal peptide (a.a. 1-18) and a propeptide sequence (a.a. 19-20) that are removed posttranslationally to yield the mature (a.a. 21-255) enzyme.

特异性

Clone AHN-11 reacted specifically with neutrophil cathepsin G, but not the homologous neutrophil neutral proteases, elastase, proteinase 3, or esterase N (Skubitz, K.M., et al. (1989). J. Leukoc. Biol. 46(2):109-118).

免疫原

Human neutrophils.

应用

Research Category
Cell Structure
Research Sub Category
Inflammation & Autoimmune Mechanisms
Western Blotting Analysis: A representative lot detected cathepsin G in the primary granule fraction from human neutrophils (Stroncek D.F., et al. (2005). J. Transl. Med. 3:36).
Immunocytochemistry Analysis: Clone AHN-11 hybridoma culture supernatant immunostained ethanol-fixed human neutrophils, but not other peripheral blood cells, including platelets, lymphocytes, and eosinophils. Faint immunostaining of some monocytes was occasionally detected (Skubitz, K.M., et al. (1989). J. Leukoc. Biol. 46(2):109-118).
ELISA Analysis: A representative lot captured cathepsin G from human neutrophil granule extract. The captured cathepsin G served to detect the presence of serum cathepsin G autoantibody levels in patients serum samples (Ellerbroek, P.M., et al. (1994). J. Clin. Pathol. 47(3):257-262).
Radioimmunoassay Analysis: Clone AHN-11 hybridoma culture supernatant bound to well surface coated with dried human neutrophils, but not platelets or erythrocytes by solid-phase radioimmunoassay (Skubitz, K.M., et al. (1989). J. Leukoc. Biol. 46(2):109-118).
Radioimmunoassay Analysis: Clone AHN-11 hybridoma culture supernatant bound to well surface coated with content of primary granule, but not secondary granule, plasma membrane, or cytoplasm fraction from neutrophils by solid-phase radioimmunoassay (Skubitz, K.M., et al. (1989). J. Leukoc. Biol. 46(2):109-118).
Radioimmunoassay Analysis: Clone AHN-11 hybridoma culture supernatant reacted with purified human cathepsin G, but not elastase, EPO, esterase N, proteinase 3, plamin, kallidrein, lactoferrin, MBP, or thrombin by solid-phase radioimmunoassay (Skubitz, K.M., et al. (1989). J. Leukoc. Biol. 46(2):109-118).
This Anti-Cathepsin G Antibody, clone AHN-11, Ascites Free is validated for use in Immunocytochemistry, Western Blotting, ELISA, Radioimmunoassay for the detection of Cathepsin G.

质量

Evaluated by Immunocytochemistry in HL60 cells.

Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected Cathepsin G in HL60 human promyelocytic leukemia cells.

目标描述

28.84 kDa (prepro-form; a.a. 1-255), 26.94 kDa (pro-form; a.a. 19-255), 26.76 kDa (active form; a.a. 21-255) calculated.

联系

Replaces: MAB1054

外形

Protein G purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆AHN-11, monoclonal
species reactivityhuman
technique(s)ELISA: suitable
immunocytochemistry: suitable
radioimmunoassay: suitable
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_001902
UniProt登记号P08311

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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