Anti-GLEPP1/PTPRO Antibody, clone 5C11

产品说明

一般描述

Receptor-type tyrosine-protein phosphatase O (EC 3.1.3.48; UniProt Q16827; also known as Glomerular epithelial protein 1, Osteoclastic transmembrane protein-tyrosine phosphatase, Protein tyrosine phosphatase U2, PTP-OC, PTP phi, PTP-U2, PTPase U2, R-PTP-O) is encoded by the PTPRO (also known as GLEPP1, NPHS6, PTPROT, PTPU2) gene (Gene ID 5800) in human. Podocytes are complex neuron-like post-mitotic cells adherent to the underlying glomerular basement membrane via foot processes that must contiguously cover the filtration surface area to maintain the normal filtration barrier. Glomerular epithelial protein 1 (GLEPP1) is a podocyte receptor membrane protein tyrosine phosphatase located on the apical cell membrane of visceral glomerular epithelial cell (VGEC) foot processes and is commonly used as a podocyte marker for assessing podocyte density. Altered GLEPP1 staining patterns are reported in kidney sections from individuals with congenital nephrotic syndrome of the Finnish type (CNF), minimal-change nephropathy (MCN), and Hodgkin’s disease. GLEPP1 is initially produced with a signal peptide sequence (a.a. 1-29), the removal of which yields the mature protein with a large extracellular region (a.a. 30-822), followed by a transmembrane segment (a.a. 823-843) and a cytoplasmic domain (a.a. 844-1216).

特异性

Clone 5C11 recognizes GLEPP1 (PTPRO) in human podocytes and neurons, but not GLEPP1 spliced isoforms lacking Fibronectin type-III domains 1-5 in their extracellular region in many other cells. Clone 5C11 detected a 200 kD protein band in glomerular extracts by Western blotting under both reducing and nonreducing conditions and immunostained only visceral glomerular epithelial cell (VGEC) in human renal cortex tissues by both fluorescent and non-fluorescent immunohistochemistry (Sharif, K., et al. (1998). Exp. Nephrol. 6(3):234-244).

免疫原

GST-tagged recombinant fragment corresponding to the Fibronectin type-III domains 1-5 of human GLEPP1/PTPRO.
Epitope: Fibronectin type-III domains 1-5.

应用

Detect GLEPP1/PTPRO using this Anti-GLEPP1/PTPRO Antibody, clone 5C11 validated for use in Immunohistochemistry, Immunofluorescence, Electron Microscopy.
Immunohistochemistry Analysis: A representative lot detected a loss of GLEPP1 immunoreactivity as a measure of podocyte density reduction (podocyte depletion) in kidney tissues with transplant glomerulopathy using formalin-fixed, paraffin-embedded kidney sections (Yang, Y., et al. (2015). J. Am. Soc. Nephrol. 26(6):1450-1465).
Immunofluorescence Analysis: Prior to purification, clone 5C11 hybridoma culture supernatant detected GLEPP1 (PTPRO) immunoreactivity predominantly on visceral glomerular epithelial cell (VGEC) foot processes along the glomerulus (GBM) in manthanol-fixed, adult human kidney cryosections, while altered GLEPP1 staining patterns were seen in kidney sections from individuals with congenital nephrotic syndrome of the Finnish type (CNF), minimal-change nephropathy (MCN), or Hodgkin’s disease (Sharif, K., et al. (1998). Exp. Nephrol. 6(3):234-244).
Electron Microscopy Analysis: Prior to purification, clone 5C11 hybridoma culture supernatant detected GLEPP1 (PTPRO) immunoreactivity at the apical aspect of the foot processes and the cell membrane of larger processes in paraformaldehyde-fixed, paraffin-embedded normal human adult kidney sections, while GLEPP1 immunoreactivity was seen redistributed from glomerulus (GBM) on the apical cell membrane of VGECs to microvilli on kidney sections from individuals with congenital nephrotic syndrome of the Finnish type (CNF) or minimal-change nephropathy (MCN) (Sharif, K., et al. (1998). Exp. Nephrol. 6(3):234-244).

质量

Evaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GLEPP1/PTPRO in human kidney tissue.

目标描述

200 kDa calculated.

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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