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Anti-IRF-9 Antibody, clone 6F1-H5

品牌
Sigma-Aldrich
货号
MABS1920
规格纯度
clone 6F1-H5, from mouse
参考价格
1415.25 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-IRF-9 Antibody, clone 6F1-H5
Interferon regulatory factor 9, IFN-alpha-responsive transcription factor subunit, ISGF3 p48 subunit, Interferon-stimulated gene factor 3 gamma, ISGF-3 gamma, Transcriptional regulator ISGF3 subunit gamma
产品介绍:

产品说明

一般描述

Interferon regulatory factor 9 (UniProt: Q61179; also known as IRF-9, IFN-alpha-responsive transcription factor subunit, ISGF3 p48 subunit, Interferon-stimulated gene factor 3 gamma, ISGF-3 gamma, Transcriptional regulator ISGF3 subunit gamma) is encoded by the Irf9 (also known as Isgf3g) gene (Gene ID: 16391) in murine species. IRF-9 is a transcription factor that mediates signaling by interferon-alpha and beta. It contains 1 IRF tryptophan pentad repeat DNA-binding domain Following binding of these interferons to cell surface receptors, JAK kinases (Tyk2 ad JAK1) are activated that lead to tyrosine phosphorylation of STAT1 and STAT2. IRF-9 then associates with phosphorylated STAT1:STAT2 dimer to form the interferon-stimulated gene factor 3 (ISFG3) complex that translocates to the nucleus and binds to the interferon stimulated response element (ISRE) to activate the transcription of interferon-stimulated genes. In mice, ablation of IRF-9 is shown to block the proliferation and migration of vascular smooth muscle cells (VSMC) and attenuates intimal thickening in response to injury. On the other hand, IRF-9 gain-of-function is reported to promote VSMC proliferation and migration that can aggravates arterial narrowing. (Ref.: Zhang, SM et al (2015). Nat Commun. 6:6882).

特异性

Clone 6F1-H5 detects interfereon regulatory factor 9 (IRF-9) of 51 kDa in murine species. May also detect shorter variants of IRF-9.

免疫原

His-tagged full length recombinant longer version (465 amino acids) of murine Interferon regulatory factor 9.

Epitope: N-terminus

应用

Research Category
Signaling
Chromatin Immunoprecipitation: Chromatin Immunoprecipitation was performed on a representative lot with wild -type or IRF9 knock-out bone marrow derived marcrophages (uninduced or treated with IFN beta). (Data courtesy of Prof. Thomas Decker, Ph.D., University of Vienna, Austria).
Anti-IRF-9, clone 6F1-H5, Cat. No. MABS1920, is a mouse monoclonal antibody that detects Interferon regulatory factor 9 (IRF-9). It has been tested for use in chromatin Immunoprecipitation and Western Blotting.

质量

Evaluated by Western Blotting in mouse spleen tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected IRF-9 in 10 µg of mouse spleen tissue lysate.

目标描述

~51 kDa observed. 44.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆6F1-H5, monoclonal
species reactivitymouse
should not react with human
包装antibody small pack of 25 μg
technique(s)ChIP: suitable
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_001152889
UniProt登记号Q61179
运输ambient

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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