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Anti-ING4 Antibody, clone BTIM-4

品牌
Sigma-Aldrich
货号
MABE1156
规格纯度
clone BTIM-4, from mouse
参考价格
5688.25 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-ING4 Antibody, clone BTIM-4
Inhibitor of growth protein 4, ING4, p29ING4
产品介绍:

产品说明

一般描述

Inhibitor of growth protein 4 (UniProt Q9UNL4; also known as Brain my036 protein, Candidate tumor suppressor p33 ING1 homolog, p29ING4) is encoded by the ING4 gene (ORF My036; Gene ID 51147) in human. The inhibitor of growth (ING) family tumor suppressor ING4 is a transcription regulator that exerts its function via chromatin remodeling. ING4 to targets the trimethylated lysine 4 of histone H3 (H3K4me3) via its conserved plant homeodomain (PHD) finger motif and is shown to associate with the HBO1/JADE histone acetyltransferase (HAT) complex. ING4 is shown to play a role in many cancer-related cellular processes, including cell proliferation, apoptosis, migration, angiogenesis, contact inhibition, DNA damage response, and hypoxia. Both gene deletion and reduced ING4 expression are reported in cancers of diverse tissue types, although ING4 knockout by itself does not cause increased spontaneous tumor formation in mice. Immunohistochemistry analysis of breast cancer tissues shows that 34% of tumors expressed undetectable to low levels of ING4. In addition, ING4 downregulation inversely correlates with an increase in NF-κB activation and NF-κB-dependent genes expression in primary breast tumors.

特异性

Clone BTIM-4 reacts with human and murine ING4, but not human ING1, ING2, or ING5 (Byron, S.A., et al. (2012). PLoS One. 7(10):e46823). Expected to react with human ING4 isoform 1 (ING4_v1) and isoform 8 (deltaEx6A). Reactivity toward human ING4 spliced isoform 2 to 7 has not been tested.

免疫原

Epitope: N-terminal half
His-tagged recombinant protein corresponding to the N-terminal half of human ING4.

应用

Western Blotting Analysis: A representative lot detected endogenous ING4 in MCF10A and T47D cells, as well as down-regulated ING4 in ING4 shRNA-transfected MCF10A cells (Byron, S.A., et al. (2012). PLoS One. 7(10):e46823).
Western Blotting Analysis: A representative lot detected full-length and degradation fragments of exogenously overexpressed human ING4 in HEK-293T, MCF10A, and T47D cells (Byron, S.A., et al. (2012). PLoS One.;7(10):e46823).
Immunocytochemistry Analysis: A representative lot detected both endogenous and exogenously overexpressed ING4 in MCF10A and T47D cells, as well as down-regulated ING4 in ING4 shRNA-transfected MCF10A cells (Byron, S.A., et al. (2012). PLoS One. 7(10):e46823).
Immunohistochemistry Analysis: A representative lot detected lower ING4 immunoreactivity in more advanced breast tumor tissue samples (Byron, S.A., et al. (2012). PLoS One. 7(10):e46823).
Note: For Western blotting application, the use of nuclear extracts will yield a stronger target band than whole cell lysates.
Anti-ING4 Antibody, clone BTIM-4 is an antibody against ING4 for use in Western Blotting, Immunocytochemistry, Immunohistochemistry.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in MCF-10A breast carcinoma cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected ING4 in 10 µg of MCF-10A human breast epithelial cell lysate.

目标描述

~22 kDa observed

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆BTIM-4, monoclonal
species reactivityhuman, mouse
technique(s)immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_001121054
UniProt登记号Q9UNL4
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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