InSolution PD 98059-CAS 167869-21-8-Calbiochem

产品说明

一般描述

Selective and cell-permeable inhibitor of MAP kinase kinase (MEK) that acts by inhibiting the activation of MAP kinase and subsequent phosphorylation of MAP kinase substrates. Pretreatment of PC-12 cells with PD 98059 completely blocks the 4-fold increase of MAP kinase activity produced by nerve growth factor (NGF; IC₅₀ = 2 µM); however, it has no effect on NGF-dependent tyrosine phosphorylation of the pp140^trk receptor or its substrate Shc and does not block NGF-dependent activation of PI 3-kinase. Inhibits cell growth and reverses the phenotype of ras-transformed BALB 3T3 mouse fibroblasts and rat kidney cells.

包装

1 ml in Glass bottle
Packaged under inert gas

生化/生理作用

Cell permeable: yes
Target IC₅₀: 2 µM in completely blocking the 4-fold increase in MAP kinase activity produced by nerve growth factor in PC12 cells
Primary Target
MAP kinase kinase (MEK)
Product does not compete with ATP.
Reversible: yes

警告

Toxicity: Irritant (B)

外形

A 5 mg/ml solution of PD 98059 (Cat. No. 513000) in anhydrous DMSO.

重悬

Following initial thaw, aliquot and freeze.

其他说明

Kültz, D., et al. 1998. J. Biol. Chem.273, 13645.
Dudley, D.T., et al. 1995. Proc. Natl. Acad. Sci. USA92, 7686.
Langlois, W.J., et al. 1995. J. Biol. Chem.270, 25320.
Pang, L., et al. 1995. J. Biol. Chem.270, 13585.
Waters, S.B., et al. 1995. J. Biol. Chem.270, 20883.



Selected Citations
Lee, J., et al. 2009. Cell Stem Cell5, 76.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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