产品说明
一般描述
G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and λ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands.
特异性
Recognizes Gαi3 and Gα0 G-proteins.
免疫原
synthetic peptide corresponding to the C-terminal region of Gαi3.
应用
Anti-Gα i3 antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 4μL using solubilized mouse brain membrane lysates and immunoblotting at a working dilution of 1:2000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates.
生化/生理作用
Gi α 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-γ activation and Ca+2 mobilization in hepatocytes. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βλ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βλ-subunit complex.
外形
Solution of whole antiserum diluted in PBS, pH 7.4.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
MDL编号 | MFCD00239679 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 200 |
偶联物 | unconjugated |
抗体形式 | whole antiserum |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | buffered aqueous solution |
分子量 | antigen ~40 kDa |
species reactivity | mouse, hamster, rat, human |
technique(s) | immunoprecipitation (IP): suitable western blot: 1:1,000-1:2,000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates |
UniProt登记号 | P08754 |
运输 | wet ice |
储存温度 | −20℃ |
Gene Information | human ... GNAI3(2773) mouse ... Gnai3(14679) rat ... Gnai3(25643) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich