产品说明
一般描述
G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and γ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands. The αz subunit is myristoylated near the N-terminus.
免疫原
synthetic peptide corresponding to the C-terminal region of Gαx/z.
应用
Anti-Gαx/z antibody produced in rabbit is suitable for western blot analysis at a working dilution of 1:100-1:200 using plasma membrane fractions.
生化/生理作用
The gene GNAZ (guanine nucleotide binding protein (G protein), αz polypeptide) encodes an α subunit of guanine nucleotide binding proteins. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βγ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βγ-subunit complex. When coupled to phospholipase C, the α subunits may have a role in pertussis toxin-insensitive pathways.
基本信息
MDL编号 | MFCD01092353 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 200 |
偶联物 | unconjugated |
抗体形式 | whole antiserum |
antibody product type | primary antibodies |
克隆 | polyclonal |
包含 | 0.1% sodium azide |
species reactivity | mammals |
technique(s) | western blot: 1:100-1:200 using plasma membrane fractions |
运输 | dry ice |
储存温度 | −20℃ |
Gene Information | human ... GNAZ(2781) mouse ... Gnaz(14687) rat ... Gnaz(25740) |
Sigma-Aldrich