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Anti-VSV-G-Peroxidase antibody, Mouse monoclonal

抗-VSV糖蛋白单克隆抗体

品牌
Sigma-Aldrich
货号
A5977
规格纯度
1.0-1.5 mg/mL, clone P5D4, purified from hybridoma cell culture
参考价格
6452.46 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-VSV-G-Peroxidase antibody, Mouse monoclonal
Monoclonal Anti-VSV Glycoprotein
抗-VSV糖蛋白单克隆抗体
产品介绍:

产品说明

一般描述

Monoclonal Anti-VSV-G-Peroxidase is a lyophilized preparation of the purified immunoglobulin fraction of monoclonal Anti-VSV-G (mouse IgG1 isotype) isolated from ascites fluid of the P5D4 hybridoma, conjugated to horseradish peroxidase (HRP). The antibody is derived from the P5D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a synthetic peptide. Vesicular Stomatitis Virus Glycoprotein (VSV-G) exists as a trimer and comprises of four domains, among which domain III is a pleckstrin homology (PH) domain.

特异性

该抗体可识别包含VSV糖蛋白的五个羧基末端氨基酸的表位。在受感染的细胞中,该抗体将未成熟型VSV-G定位在粗糙内质网(RER)和高尔基复合体潴泡中,将成熟型VSV-G定位在细胞表面和出芽病毒中。该抗体不会染色缺少膜和细胞质结构域的分泌型VSV-G。该抗体可用于微注射抗体、免疫印迹、免疫沉淀、免疫细胞化学和免疫电镜法研究新合成的VSV-G在向质膜和细胞表面转移过程中细胞质结构域的作用。该抗体可用于对以VSV-G羧基末端标记的外源性导入结构进行检测、免疫沉淀和免疫细胞化学染色。该标记不会干扰所研究蛋白质的功能,可被P5D4抗体特异性识别,而不与任何内源性蛋白质发生交叉反应。
VSV-G tag sequence (YTDIEMNRLGK) on VSV-G tagged fusion proteins when expressed N- or C-terminal to the fusion protein. Recognizes native and denatured forms of VSV-G tagged proteins.

免疫原

KLH标记的含有水泡性口炎病毒糖蛋白(VSV-G)的15个羧基末端氨基酸(497-511)的合成肽。

应用

Monoclonal Anti-VSV-G−Peroxidase antibody produced in mouse may be used immunoblotting.

生化/生理作用

Vesicular Stomatitis Virus Glycoprotein (VSV-G) amino acid sequence YTDIEMNRLGK, corresponding to amino acids 501-511 has been widely used as an epitope tag in expression vectors, enabling the expression of proteins as VSV-G tagged fusion proteins. It constitutes an attractive model to study maturation and intracellular transport of membrane proteins. VSV-G mediates attachment of VSV to the cell surface and induces pH-dependent fusion between viral and target membranes. Besides, its cytoplasmic domain contains information for several intracellular sorting steps, which include efficient export from the endoplasmic reticulum, basolateral delivery and endocytosis..VSV-G is transported through stacks in the Golgi cisternae.

外形

Supplied as a lyophilized powder. After reconstitution,the solution contains 1% BSA and 0.05% MIT in 0.01 M sodium phosphate buffered saline, pH 7.4.

重悬

Reconstitution with 0.5 mL water results in a solution of 0.01 M sodium phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% MIT.

储存及稳定性

Stored the lyophilized product at 2-8 ℃. After reconstitution, for extended storage, freeze in working aliquots at –20 ℃. For continuous use, the solution may be store at 2-8 ℃ for up to 1 month. Working dilutions should be discarded. Avoid repeated freeze-thaw.

免责声明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.56

产品性质

质量水平200
生物来源mouse
偶联物peroxidase conjugate
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆P5D4, monoclonal
形式lyophilized powder
浓度1.0-1.5 mg/mL
technique(s)western blot: 1:1,000 using 20-50 ng of a purified VSV-G tagged fusion protein
同位素/亚型IgG1
运输wet ice
储存温度2-8℃

安全信息

象形图GHS07
警示用语:Warning
危险声明H317
预防措施声明P280 - P302 + P352
危险分类Skin Sens. 1
储存分类代码13 - Non Combustible Solids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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