Anti-VGAT Antibody, clone 2E6 ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

特异性

Clone 2E6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects vesicular GABA transporter. It targets an epitope within 15 amino acids from the C-terminal, cytoplasmic domain.

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal, cytoplasmic domain of human Vesicular GABA transporter.

应用

Quality Control Testing
Evaluated by Western Blotting in Human Brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected VGAT in Human Brain tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 Dilution from a representative lot detected VGAT in Y79 cell line.
Affinity Binding Assay Analysis: A representative lot of this antibody bound VGAT peptides with a KD of 5.3 x 10^-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected VGAT in human cerebellum tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-VGAT, clone 2E6 ZooMAb^®, Cat. No. ZRB1158, is a recombinant Rabbit monoclonal antibody that specifically targets VGAT and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Vesicular inhibitory amino acid transporter (UniProt: Q9H598; also known as GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, hVIAAT, VGAT) is encoded by the SLC32A1 (also known as VGAT, VIAAT) gene (Gene ID: 140679) in human. VGAT is a multi-pass membrane protein with six cytoplasmic domains, ten transmembrane domains, and five vesicular lumenal domains. It is shown to be highly concentrated in the nerve endings of GABAergic neurons and in glycinergic nerve endings. It transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. It is also expressed throughout the horizontal cell tips at photoreceptor terminals in retina. VGAT expression has been reported in pancreatic islets of rat, however, its expression in human pancreatic islets is none to very low. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chessler, SD., et al. (2002). Diabetes. 51(6); 1763-1771).

外形

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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