产品说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 4O5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Bone morphogenetic protein 2 (BMP-2). It targets an epitope within the C-terminal half.
免疫原
GST/His-tagged recombinant fragment corresponding to 101 amino acids from the C-terminal region of human BMP-2.
应用
Anti-BMP-2, clone 4O5 ZooMAb, Cat. No. ZRB1489, is a recombinant Rabbit monoclonal antibody that specifically targets BMP-2 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.
Affinity Binding Assay: A representative lot of this antibody bound human recombinant BMP-2 with a KD of 1.8 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected BMP-2 in one million HepG2 cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected BMP-2 in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Cyclin-dependent kinase 6 (UniProt: Q00534; also known as EC:2.7.11.22, Cell division protein kinase 6, Cdk6, Serine/threonine-protein kinase PLSTIRE) is encoded by the CDK6 (also known as CDKN6) gene (Gene ID: 1021) in human. CDK6 is a ubiquitously expressed serine/threonine-protein kinase that is involved in the control of the cell cycle and differentiation. Its kinase domain is localized to amino acids 13-300 and its kinase activity is observed only in the nucleus. It associates with D-type cyclins (D1, D2 and D3), and the resulting complexes enable the cell to pass from the G1 phase to the S phase. Its binding to cyclin promotes its activation by phosphorylation at threonine 177. CDK6 is considered as a versatile player in transcriptional regulation and differentiation, participating in the integration of signaling networks and controlling changes in gene expression and epigenetic status, which contribute to cell fate determination. Over-expression of CDK6 has been reported in some leukemias and malignancies, including sarcoma, glioma, breast tumors, lymphoma, and melanoma. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tigan., AS et al. (2016). Oncogene 35(24); 3083-3091).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
生物来源 | rabbit (recombinant) |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 4O5, monoclonal recombinant monoclonal |
产品线 | ZooMAb® learn more |
形式 | lyophilized |
分子量 | calculated mol wt 44.7 kDa observed mol wt 38 kDa |
species reactivity | human |
包装 | antibody small pack of 25 μL |
环保替代产品特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | affinity binding assay: suitable flow cytometry: suitable immunocytochemistry: suitable western blot: suitable |
同位素/亚型 | IgG |
环保替代产品分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 1 |
Sigma-Aldrich