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位置:首页 > 品牌 > Sigma-Aldrich > Anti-BMP-2 Antibody, clone 4O5 ZooMAb® Rabbit Monoclonal

Anti-BMP-2 Antibody, clone 4O5 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1489
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-BMP-2 Antibody, clone 4O5 ZooMAb® Rabbit Monoclonal
BMP-2A
Bone morphogenetic protein 2
Bone morphogenetic protein 2A
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 4O5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Bone morphogenetic protein 2 (BMP-2). It targets an epitope within the C-terminal half.

免疫原

GST/His-tagged recombinant fragment corresponding to 101 amino acids from the C-terminal region of human BMP-2.

应用

Anti-BMP-2, clone 4O5 ZooMAb, Cat. No. ZRB1489, is a recombinant Rabbit monoclonal antibody that specifically targets BMP-2 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.
Affinity Binding Assay: A representative lot of this antibody bound human recombinant BMP-2 with a KD of 1.8 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected BMP-2 in one million HepG2 cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected BMP-2 in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Cyclin-dependent kinase 6 (UniProt: Q00534; also known as EC:2.7.11.22, Cell division protein kinase 6, Cdk6, Serine/threonine-protein kinase PLSTIRE) is encoded by the CDK6 (also known as CDKN6) gene (Gene ID: 1021) in human. CDK6 is a ubiquitously expressed serine/threonine-protein kinase that is involved in the control of the cell cycle and differentiation. Its kinase domain is localized to amino acids 13-300 and its kinase activity is observed only in the nucleus. It associates with D-type cyclins (D1, D2 and D3), and the resulting complexes enable the cell to pass from the G1 phase to the S phase. Its binding to cyclin promotes its activation by phosphorylation at threonine 177. CDK6 is considered as a versatile player in transcriptional regulation and differentiation, participating in the integration of signaling networks and controlling changes in gene expression and epigenetic status, which contribute to cell fate determination. Over-expression of CDK6 has been reported in some leukemias and malignancies, including sarcoma, glioma, breast tumors, lymphoma, and melanoma. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tigan., AS et al. (2016). Oncogene 35(24); 3083-3091).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆4O5, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 44.7 kDa
observed mol wt 38 kDa
species reactivityhuman
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 1

Sigma-Aldrich

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