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位置:首页 > 品牌 > Sigma-Aldrich > Anti-Desmin Antibody, clone 1H16, ZooMAb® Rabbit Monoclonal

Anti-Desmin Antibody, clone 1H16, ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1178
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Desmin Antibody, clone 1H16, ZooMAb® Rabbit Monoclonal
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 1H16 is a ZooMAb , rabbit recombinant monoclonal antibody that specifically detects Desmin. It targets an epitope within the C-terminal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 64 amino acids from the C-terminal region of human Desmin.

应用

Anti-Desmin, clone 1H16 ZooMAb , Cat. No. ZRB1178, is a highly specific rabbit recombinant monoclonal antibody that targets Desmin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Desmin in lysates from C2C12 cells and rat heart tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Desmin in human skeletal muscle and rat heart tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Desmin in human skeletal muscle cells (SkMC) and C2C12 cells.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

目标描述

Desmin (UniProt: P17661) is encoded by the DES (also known as CDCD3, CMD1F, CSM1) gene (Gene ID: 1674) in human. Desmin is a homopolymeric protein that is classified as a muscle-specific type III intermediate filament. It is shown to be essential for proper muscular structure and function. It plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. It also acts as a sarcomeric microtubule-anchoring protein that specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Desmin can undergo phosphorylation at multiple serines by CDK1. Phosphorylation at serine 60 by Aurora B and threonine 76 by ROCK1 is reported to contribute to efficient separation of Desmin intermediate filaments during mitosis. Mutations in DES gene have been linked to myofibrillar myopathy that is characterized at ultrastructural level by disintegration of the sarcomeric Z disk and myofibrils, and replacement of the normal myofibrillar markings by small dense granules, or larger hyaline masses, or amorphous material. It leads to skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, and restrictive heart failure. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1H16, recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 53.54 kDa
observed mol wt ~53 kDa
species reactivityhuman, rat, mouse
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable (peptide)
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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