产品说明
一般描述
Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.
生化/生理作用
Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.
单位定义
One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37℃ in 50 mM HEPES, pH 7.5 containing 10 mM CaCl2.
外形
Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)
产品性质
质量水平 | 200 |
生物来源 | human |
重组 | expressed in insect cells |
测定 | ≥90% (SDS-PAGE) |
形式 | lyophilized powder |
效能 | >0.004 units/mg |
specific activity | ≥40 units/μg |
包装 | pkg of 5 μg |
UniProt登记号 | P10144 |
运输 | wet ice |
储存温度 | −20℃ |
Gene Information | human ... GZMB(3002) |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich