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Anti-GABA A Receptor β2/3 Antibody

品牌
Sigma-Aldrich
货号
05-474
规格纯度
clone 62-3G1, Upstate®, from mouse
参考价格
6694.05 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-GABA A Receptor β2/3 Antibody
GABA(A) receptor subunit alpha-1, gamma-aminobutyric acid (GABA) A receptor, alpha 1, gamma-aminobutyric acid A receptor, alpha 1
产品介绍:

产品说明

一般描述

The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 3 different isoforms of the beta subunit, and receptor subunit expression varies during development. Additionally, GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 and beta 2/3 subunits increase upon chronic exposure to alcohol, and may be potential therapeutic targets for alcohol dependency. The beta 2 subunit is required for receptor assembly and surface expression, while mice with an inactivated beta3 subunit exhibit epilepsy and cleft palate.

特异性

Specific for the GABAA Receptor β2 subunit, MW ~55 kDa and β3 subunit, MW ~57 kDa.

免疫原

Affinity-purified GABAA receptor isolated from bovine brain. Clone 62-3G1

应用

Immunoprecipitation: 4 μg of a previous lot immunoprecipitated the GABAA Receptor β2 and β3 subunits from 500 μg of rat brain microsomal tissue extract. Due to interference of the IgG heavy chain, the immunoprecipitated GABAA receptor complex was confirmed using a polyclonal anti-GABAA receptor α1 subunit (Catalog # 06-868).
Immunohistochemistry: This antibody has been reported to detect the GABAA Receptor β2 and β3 subunits in paraffin embedded and frozen rat brain sections which had been fixed with 4% paraformaldehyde or 0.1% glutaraldehyde and permeabilized with Triton X-100.
Anti-GABA A Receptor β2/3 Antibody, is an antibody against GABA A Receptor β2/3 for use in WB, IP, IH.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

质量

Evaluated by western blot on rat brain microsomal preparation.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected the GABAA Receptor β2 and β3 subunits from 20 μg of rat brain microsomal preparation (Catalog # 12-144).

目标描述

Varies

外形

Format: Purified
Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2°C to 8°C from date of receipt.

分析说明

Control
Brain tissue

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆62-3G1, monoclonal
species reactivityhuman, rat, bovine
manufacturer/tradenameUpstate®
technique(s)immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG
NCBI登记号XM_583106
XM_612515
UniProt登记号P14867
运输wet ice
Gene Informationhuman ... GABRA1(2554)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

Sigma-Aldrich

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