产品说明

一般描述

该抗体对日本血吸虫 谷胱甘肽-S-转移酶的天然型和变性-还原型均具有特异性 。抗 GST 可用于各种免疫分析，以鉴定 GST 融合蛋白的表达。
Glutathione-S-Transferase (GST) belongs to a family of Phase II detoxification enzymes which are involved in catalyzing the conjugation of glutathione (GSH) with many exogenous and endogenous electrophilic substances. GST is of two kinds- membrane-bound microsomal and cytosolic. The cytosolic GSTs are made of six classes: α, μ, ω, π, θ, and ζ.

特异性

Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.

免疫原

日本血吸虫 的重组 GST 在 大肠杆菌中表达 。

应用

Anti-Glutathione-S-Transferase (GST)-Peroxidase Conjugate antibody has been used in

• immunoblotting
• affinity pull-down assay
• western blotting
• enzyme linked immunosorbent assay (ELISA)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

生化/生理作用

Glutathione-S-Transferase (GST) are involved in protecting cellular macromolecules from the reactive electrophiles. The μ and π transferases regulate the mitogen-activated protein (MAP) kinase pathway. GSTs help in building up cellular resistance against microbial antibiotic, herbicides, insecticides, chemotherapy agents and drugs.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

制备说明

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.46

产品性质

生物来源	rabbit
质量水平	200
偶联物	peroxidase conjugate
抗体形式	IgG fraction of antiserum
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen 27.5 kDa
technique(s)	direct ELISA: 1:10,000 western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST
运输	dry ice
储存温度	−20℃

安全信息

象形图
警示用语：	Danger
危险声明	H317 - H334
预防措施声明	P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
危险分类	Resp. Sens. 1 - Skin Sens. 1
储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable