产品说明
一般描述
Dihydropyridine Receptor (DHPR) is a heteropentamer calcium ion channel and comprises of α1,α2/δ,β and γ subunits. It is localized in the skeletal muscle in the t-tubule membrane. The α2subunit of DHPR is a glycosylated subunit and is anchored on the membrane.
特异性
Reacts specifically with the α2 subunit of the DHP receptor. Strong reactivity is observed with the α2 subunit in rat, mouse, guinea pig, rabbit skeletal and cardiac muscle, and rat brain and lung tissue. Weaker reactivity is observed with rat kidney, liver and spleen tissues. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.
免疫原
Rabbit dihydropyridine (DHP) receptors, (α2 subunit)
应用
Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse has been used in:
生化/生理作用
Dihydropyridine Receptor (DHFR) α1 subunit is the voltage-sensor. DHPR interacts with ryanodine receptor (RyR) and also mediates excitation-contraction (EC) coupling in cardiac and skeletal muscles. Mutation in the DHPR (E1014K) affects its affinity and selectivity towards calcium permeation. Additionally, it also plays a key role in the synaptic transmission.
外形
Solution in phosphate buffered saline containing 0.05% sodium azide.
其他说明
This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
MDL编号 | MFCD01323189 |
NACRES | NA.41 |
产品性质
质量水平 | 200 |
生物来源 | mouse |
偶联物 | unconjugated |
抗体形式 | ascites fluid |
antibody product type | primary antibodies |
克隆 | 20A, monoclonal |
形式 | buffered aqueous solution |
分子量 | antigen 143 kDa (reduced) antigen 220 kDa (non-reduced) |
species reactivity | guinea pig, rabbit, mouse, rat |
technique(s) | immunohistochemistry (frozen sections): 1:500 western blot: 1:500 |
同位素/亚型 | IgG2a |
UniProt登记号 | O08532 |
运输 | dry ice |
储存温度 | −20℃ |
Gene Information | mouse ... Cacna2d1(12293) rat ... Cacna2d1(25399) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich