产品说明
一般描述
β-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.
应用
β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.
在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解
比较不同酶性能的新技术文章
了解更多
关于Sigma R&D产生的最新应用数据，以优化不同来源的酶对不同药物类别的水解条件，并使用色谱纯化酶降低酯酶活性的影响，从而将6-MAM转化为吗啡
有效水解类固醇葡糖苷酸。
包装
25000, 100000 units in poly bottle
单位定义
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 ℃ at the pH 6.8 (30 min assay).
外形
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Highly purified solution in 50% glycerol
基本信息
| 酶学委员会(EC)编号 | 3.2.1.31 ( BRENDA | IUBMB ) |
| EC 号 | 232-606-8 |
| MDL编号 | MFCD00130629 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli |
| 形式 | aqueous glycerol solution |
| specific activity | >20,000,000 units/g protein |
| 分子量 | 74 kDa |
| 浓度 | >1 mg/mL |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H317 - H334 |
| 预防措施声明 | P261 - P280 - P342 + P311 |
| 危险分类 | Resp. Sens. 1 - Skin Sens. 1 |
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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