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位置:首页 > 品牌 > Sigma-Aldrich > PhosphoDetect Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb

PhosphoDetect Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb

品牌
Sigma-Aldrich
货号
506119
规格纯度
liquid, Calbiochem®
参考价格
7365.52 *本价格含增值税费
促销
服务
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数量
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产品名称:
PhosphoDetect Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb
产品介绍:

产品说明

一般描述

Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect Anti-p38 MAP Kinase Rabbit pAb is validated for use in WB, ICC for the detection of p38 MAP Kinase .
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~p38 MAPK phosphorylated at Thr180 and Tyr182.
Recognizes p38α, β, and γ/ERK6 phosphorylated at Thr180 and Tyr182 in uv-treated HEK293 cells and sorbitol-treated PC12 cells.

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
Human

包装

10 tests in Plastic ampoule

应用

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

警告

Toxicity: Standard Handling (A)

外形

In Dulbecco′s PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
UV irradiated HEK293 cells or sorbitol-treated PC12 cells

其他说明

Marinissen, M.J., et al. 2001. Genes Dev.15, 535.
Nakagami, H., et al. 2001. Diabetes50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem.274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem.270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA92, 10531.
Han, J., et al. 1994. Science265, 808.
Lee, J.C., et al. 1994. Nature372, 739.
Rouse, J., et al. 1994. Cell78, 1027.
Does not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式liquid
包含≤0.1% sodium azide as preservative
species reactivityhuman, rat, mouse, canine
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型IgG
运输wet ice
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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