产品说明
一般描述
DFF exists as an inactive cytoplasmic protein until activated by apoptotic signals. DFF40, the nuclease responsible for DNA fragmentation during apoptosis, exists as a heterodimeric complex with DFF45.
免疫原
synthetic peptide corresponding to amino acids 2-21 of the N-terminal region of human DFF45/ICAD (DNA Fragmentation Factor 45/Inhibitor of Caspase-3 Activated DNase I).
应用
Anti-DFF45 antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5μg/mL using a total HeLa cell lysate.
生化/生理作用
DFF45 functions as both a chaperone, mediating the correct folding of DFF40, and an inhibitor of DFF40. DFF45 is cleaved by caspase-3 at two sites in response to apoptotic signals. This releases DFF40, the active nuclease. DFF40 seems to oligomerize, forming a large, functional complex which breaks down DNA by introducing double strand breaks. Also, DFF40 appears to interact directly with histone H1 that may stimulate its activity.
外形
Solution in phosphate buffered saline containing 0.02% sodium azide.
基本信息
| MDL编号 | MFCD02096210 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 偶联物 | unconjugated |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 45 kDa (non-apoptotic cells) |
| species reactivity | human |
| technique(s) | western blot: 0.5 μg/mL using human HeLa cell extract |
| UniProt登记号 | O00273 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... DFFA(1676) |
Sigma-Aldrich
m.cnreagent.com
