产品说明
一般描述
Interleukin-4 (IL-4) is a T cell derived chemokine that stimulates the Th2 mediated immune response and proliferation of B cells. The effects of IL-4 are mediated by two types of receptors, type I receptor consisting of IL-4Rα and common γ chain and type II receptor composed of IL-4Rα and IL-13Rα1. The signalling stimulated by IL-4R leads to activation of JAK/STAT6 and IRS-mediated PI3K/Akt pathway. Through these pathways, IL-4 is responsible for endocytic activity of macrophages, chemotaxis of leukocytes in response to inflammation, angiogenesis and regulation of nitric oxide metabolism in macrophages. Anti-tumor effects of IL-4R signaling have been reported in cancers of breast, liver and renal cells
Anti-Interleukin-4 soluble receptor specifically recognizes human cell surface IL-4R.
特异性
The antibody will neutralize human cell surface IL-4 receptor mediated-bioactivity.
免疫原
recombinant human IL-4 soluble receptor (IL-4 sR), expressed in Sf 21 insect cells.
应用
Anti-Interleukin-4 soluble receptor antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA, a working concentration of 0.5-1.0 μg/ml is recommended. The antibody is suitable for neutralization reactions at a working concentration of 5-10 μg/ml. This antibody was used for immunostaining of parasympathetic neurons from guinea pig and human neuroblastoma cells at a dilution of 1:200.
外形
Lyophilized from 51.5 μL of a 0.2μm filitered solution in PBS (pH7.4) with 5% trehalose.
制备说明
Purified using Protein G.
基本信息
MDL编号 | MFCD02096774 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | goat |
偶联物 | unconjugated |
抗体形式 | IgG fraction of antiserum |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | lyophilized powder |
species reactivity | human |
technique(s) | indirect ELISA: suitable neutralization: suitable western blot: suitable |
UniProt登记号 | P24394 |
储存温度 | −20℃ |
Gene Information | human ... IL4R(3566) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich