产品说明
特异性
Recognizes rat MMP-2. Exhibits no cross-reactivity with other MMP family members. The antibody was generated using E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain) as an immunogen.
免疫原
E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain)
Epitope: catalytic domain
应用
Research Category
Cell Structure
Western blot: 1:2,000
Immunoprecipitation: 1:500
MMP-2 [Gelatinase-A] is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen. The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, 1992) or immunoprecipitation. This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.
Optimal working dilutions must be determined by end user.
Detect MMP-2 using this Anti-MMP-2 Antibody, catalytic domain validated for use in IP & WB.
Research Sub Category
MMPs & TIMPs
联系
Replaces: 04-1048
外形
Purified immunoglobulin. Liquid in PBS, containing 0.05% sodium azide.
Format: Purified
储存及稳定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human, mouse, rat |
manufacturer/tradename | Chemicon® |
technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
NCBI登记号 | NM_004530.2 |
UniProt登记号 | P08253 |
运输 | wet ice |
Gene Information | human ... MMP2(4313) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich